Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 20mg of bovine tissues and Lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction. Note all samples have very similar yields. Purity was also tested and was consistently between 1.9 and 2.0 (not shown).
2. 在细胞中提取基因组DNA (Hela细胞系) 琼脂糖凝胶电泳分析
Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 1X106 cells of cultured HeLa cell and Lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction. Note all samples have very similar yields. Purity was also tested and was consistently between 1.8 and 2.0 (not shown).